LOCUS       sequence-51225-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1530
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        166..270
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(489..508)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttacgcatg ctaaccatga ctaactcctt ttttatcatc tctggaacac acacaatccg
       61 tgtgcaggtc cctgccagag tcgacagtat cttgttatcc gctcacaatg tcaatgttat
      121 ccgctcacat ttatgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      181 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      241 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      301 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      361 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      421 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      481 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      541 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      601 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      661 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      721 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      781 ataccaaacg acgagcgtga caccacgatg cctg
//
