LOCUS       sequence-51226-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZBTB48
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(455..475)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(500..517)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             528..550
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    565..589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(589..608)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(590..608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(692..711)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgcn tncttgtcga
       61 cgagagctcg aattcggatc cgtatccacc tttactgtca cactgaagtt ttgggcttga
      121 agctctggca cagctctacg gcctctggca ctcgcaactc cctggctgcc aggagcacct
      181 gatcccggtt ccctgaggtg agagcgaggt gaccagtgta gaaaaagtcc aacaagaggc
      241 caaagatctc agcgaagcca gcagggagga cgacactgcc ccctgagcca tccccgtaga
      301 ggctctggaa aaagtgactg cagcaggcaa ggacactcca gtgtgcctta aacaccaggc
      361 cccccacgtc cagagtggcg tcgcagtact ggcccttctc ccgctgcttg ttgagctcct
      421 gcagaaccct cacactgtgc tggacgaagg acatggattg gaagtacagg ttctcggtac
      481 ccagatctac accagaagaa tgatgatgat gatggtgcat atgtatatct ccttcttaaa
      541 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
      601 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
      661 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      721 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      781 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
      841 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
      901 gagcgtcgag atcccggaca c
//
