LOCUS       sequence-51231-e        9935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pLentiEKAR2G1
FEATURES             Location/Qualifiers
     source          1..9935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2671..2796
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3289..3522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4012..4129
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(4135..4155)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4140..4469
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4320..4455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4479..5078
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4479..4498)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4975..4995
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        5150..5453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5454..5657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5608..5628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5654..5678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5757..5774
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5784..5813
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7134..7868
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     7271..7292
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7395..7416)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7656..7675)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7938..7955
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    7997..8585
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8050..8070)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8468..8479)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8676..8909
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8981..9102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9018..9037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9072..9091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9121..9256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9288..9307)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9289..9307)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9317..9334)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9317..9333)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9326..9348)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9478..9933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9565..9584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9775..9796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacctga aagcgaaagg gaaaccagag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcctcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttgtggaatg
     4141 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4201 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4261 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4441 gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg
     4501 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4561 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4621 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     4681 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     4741 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     4801 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     4861 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     4921 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     4981 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc
     5041 gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     5101 gcccgaccga aaggagcgca cgacccaatc gataagcttg ggagttccgc gttacataac
     5161 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     5221 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     5281 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     5341 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     5401 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     5461 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     5521 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     5581 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     5641 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     5701 gttttgacct ccatagaaga caccgactct agaggatccg gagatatacc atggcacacc
     5761 atcaccacca ccacgggagc ggggagcaga aactaatatc agaggaggat cttggctcag
     5821 gttcaggatc ctccaccgac ggcatggacg agctgtacaa gggtgctagc ggaggaatgg
     5881 tgagcaaggg cgaggagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga
     5941 agatggaggg caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc
     6001 cctacgacgg caccaacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct
     6061 cctacgacat tctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg
     6121 acgacatccc caactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcacca
     6181 tgaccttcga ggacaagggc atcgtgaagg tgaagtccga catctccatg gaggaggact
     6241 ccttcatcta cgagatacac ctcaagggcg agaacttccc ccccaacggc cccgtgatgc
     6301 agaagaagac caccggctgg gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc
     6361 tgaagggcga cgtcaagcac aagctgctgc tggagggcgg cggccaccac cgcgttgact
     6421 tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc
     6481 accgcatcga gatcctgaac cacgacaagg actacaacaa ggtgaccgtt tacgagagcg
     6541 ccgtggcccg caactccgga atggcggacg aggagaagct gccgcccggc tgggagaagc
     6601 gcatgagccg cagctcaggc cgagtgtact acttcaacca catcactaac gccagccagt
     6661 gggagcggcc cagcggcaac agcagcagtg gtggcaaaaa cgggcagggg gagcctgcca
     6721 ggaagctgcg cattctgcag tcgactaggg agcaagggct tgtgcccggg tctagaggcg
     6781 gaggtggtgg aggaggtgga ggtggtggag gtggaggtgg aggtggtggc ggtggtggag
     6841 gaggaggagc tagaggcgga ggtggtggag gaggtggagg tggtggaggt ggaggtggag
     6901 gtggtggcgg tggtggagga ggaggagcca gcggcggagg tggtggagga ggtggaggtg
     6961 gtggaggtgg aggtggaggt ggtggcggtg gtggaggagg aggaaccggt gctgaccaac
     7021 tgactgaaga gtggtcgacg gtacagcgga ccgcgcctga cgtaccacgt acaccagtag
     7081 gaaaggcaaa gctttcattc caattcccgc gggcgcggga catcgcggcc gcgatggacg
     7141 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7201 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     7261 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7321 acgagctgta caagggtggc agcggtggca tggtgagcaa gggcgaggag ctgttcaccg
     7381 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     7441 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
     7501 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgatgt
     7561 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     7621 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     7681 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     7741 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     7801 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     7861 acatcgaggg taccgatatc cttcaaaaaa aacttgaaga acttgaactt gacgaatgag
     7921 atatctaatg actcgagcac caccatcacc atcaccagga tcctgcagtc tagagctagc
     7981 atatgaattc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     8041 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     8101 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     8161 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8221 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     8281 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8341 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     8401 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8461 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8521 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8581 cccgcctgga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     8641 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8701 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8881 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8941 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     9001 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9061 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     9121 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9181 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9241 ggcttttttg gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt
     9301 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     9361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     9421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg
     9481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9901 cgaattttaa caaaatatta acgtttacaa tttcc
//
