LOCUS       sequence-51238-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRDT
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(384..404)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(429..446)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             457..479
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    494..518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(518..537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(519..537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(621..640)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 attcggatcc gtatccacct ttactgtcac tcttcttgtg gcatctgaga taatttctgc
       61 ataaacagct tctctagagc ttgtgccata agaacaatgt catctccagg cttgttatat
      121 aaataacaat ttgagaacat tgtattgaag tcttctatac attctgaagc cttcgcataa
      181 tatttattct ccaagcgctt cttaattgta tttaaatcca ttgggttttt tataatggta
      241 taataatcag gcaactgtag tttcacagca tccacaggac gttgaaaggg ccatgaaaaa
      301 ctatgcttcc ataaatcctt taggacaact ttttgtagat actgaagttg atttgtcaat
      361 cgcccatttt tcttagtatt catggattgg aagtacaggt tctcggtacc cagatctaca
      421 ccagaagaat gatgatgatg atggtgcata tgtatatctc cttcttaaag ttaaacaaaa
      481 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
      541 tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      601 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      661 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      721 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      781 cctcaaccta ctactgggct gcttcctaa
//
