LOCUS       sequence-51239-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRDT
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             518..535
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(588..606)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      602..649
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(773..792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gaatactaag aaaaatgggc
      121 gattgacaaa tcaacttcag tatctacaaa aagttgtcct aaaggattta tggaagcata
      181 gtttttcatg gccctttcaa cgtcctgtgg atgctgtgaa actacagttg cctgattatt
      241 ataccattat aaaaaaccca atggatttaa atacaattaa gaagcgcttg gagaataaat
      301 attatgcgaa ggcttcagaa tgtatagaag acttcaatac aatgttctca aattgttatt
      361 tatataacaa gcctggagat gacattgttc ttatggcaca agctctagag aagctgttta
      421 tgcagaaatt atctcagatg ccacaagaag agtgacagta aaggtggata cggatccgaa
      481 ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      541 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      601 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      661 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      721 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      781 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      841 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccca aaaa
//
