LOCUS       sequence-51240-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX3
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             583..603
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             604..621
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             622..645
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             695..712
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(765..783)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      779..826
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gactgccatg gtggcataag tggcaccatt tacgagtacg gagccctcac cattgatggg
       61 gaggagtaca tccccttcaa gcagtatgct ggcaaatacg tcctctttgt caacgtggcc
      121 agctacggag gcctgacggg ccagtacatt gaactgaatg cactacagga agagcttgca
      181 ccattcggtc tggtcattct gggctttccc tgcaaccaat ttggaaaaca ggaaccagga
      241 gagaactcag agatccttcc taccctcaag tatgtccgac caggtggagg ctttgtccct
      301 aatttccagc tctttgagaa aggggatgtc aatggagaga aagagcagaa attctacact
      361 ttcctaaaga actcctgtcc tcccacctcg gagctcctgg gtacatctga ccgcctcttc
      421 tgggaaccca tgaaggttca cgacatccgc tggaactttg agaagttcct ggtggggcca
      481 gatggtatac ccatcatgcg ctggcaccac cggaccacgg tcagcaacgt caagatggac
      541 atcctgtcct acatgaggcg gcaggcagcc ctgggggtcg cagagaacct ctacttccaa
      601 tcgcaccatc atcaccacca tgattacaag gatgacgacg ataagtgagg atccgaattc
      661 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      721 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      781 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      841 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      901 gtggttacgc gcagcgtgac cgct
//
