LOCUS       sequence-51245-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RHOU
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat
       61 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat ggggcctggg
      121 gagccggggg gccgggggcg tgcggggggt gccgaggggc gcggcgtcaa gtgcgtgctg
      181 gtcggcgacg gcgcggtggg caagacgagc ctggtggtga gctacaccac caacggctac
      241 cccaccgagt acatccctac tgccttcgac aacttctccg cggtggtgtc tgtggatggg
      301 cggcccgtga gactccaact ctgtgacact gccggacagg atgaatttga caagctgagg
      361 cctctctgct acaccaacac agacatcttc ctgctctgct tcagtgtcgt gagcccctca
      421 tccttccaga acgtcagtga gaaatgggtg ccggagattc gatgccactg tcccaaagcc
      481 cccatcatcc tagttggaac gcagtcggat ctcagagaag atgtcaaagt cctcattgag
      541 ttggacaaat gcaaagaaaa gccagtgcct gaagaggcgg ctaagctgct agccgaggaa
      601 atcaaagccg cctcctacat cgagtgttca gccttgactc aaaaaaacct caaagaggtc
      661 tttgatgcag ccatcgtcgc tggcattcaa tactcggaca ctcagcaatg acagtaaagg
      721 tggatacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcacacac
      781 acaca
//
