LOCUS       sequence-51246-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RHOU
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(710..730)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca
       61 agcttgtcga cggagctcga attcggatcc gtatccacct ttactgtcat tgctgagtgt
      121 ccgagtattg aatgccagcg acgatggctg catcaaagac ctctttgagg tttttttgag
      181 tcaaggctga acactcgatg taggaggcgg ctttgatttc ctcggctagc agcttagccg
      241 cctcttcagg cactggcttt tctttgcatt tgtccaactc aatgaggact ttgacatctt
      301 ctctgagatc cgactgcgtt ccaactagga tgatgggggc tttgggacag tggcatcgaa
      361 tctccggcac ccatttctca ctgacgttct ggaaggatga ggggctcacg acactgaagc
      421 agagcaggaa gatgtctgtg ttggtgtagc agagaggcct cagcttgtca aattcatcct
      481 gtccggcagt gtcacagagt tggagtctca cgggccgccc atccacagac accaccgcgg
      541 agaagttgtc gaaggcagta gggatgtact cggtggggta gccgttggtg gtgtagctca
      601 ccaccaggct cgtcttgccc accgcgccgt cgccgaccag cacgcacttg acgccgcgcc
      661 cctcggcacc ccccgcacgc ccccggcccc ccggctcccc aggccccatg gattggaagt
      721 acaggttctc ggtacccaga tctacaccag aagaatgatg atgatga
//
