LOCUS       sequence-51247-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: JARID1C
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             506..523
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(576..594)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      590..637
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttaacttta agaaggagat atacatatgc accatcatca tcatcattct tctggtgtag
       61 atctgggtac cgagaacctg tacttccaat ccatgaatga gctagaggcc cagacgagag
      121 tgaaactgaa ctacttggac cagattgcca aattctggga aatccagggc tcctccttaa
      181 agattcccaa tgtagaacgg cggatcttgg acctctacag tctcagcaaa attgtggtgg
      241 aggaaggtgg ttatgaagct atctgcaagg accgtcggtg ggctcgggta gcccagcgcc
      301 tcaactatcc accaggcaaa aatattggct ccttgctacg ctcccactac gaacgcattg
      361 tttatcccta tgaaatgtac cagtctggag ccaaccttgt gtgtaacaca cgtccatttg
      421 ataatgagga gaaggacaag tgacagtaaa ggtggatacg gatccgaatt cgagctccgt
      481 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      541 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      601 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      661 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      721 cgcagcgtga ccgctacact tgccagcgcc cta
//
