LOCUS       sequence-51249-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Tirnauer JS, Grego S, Salmon ED, Mitchison TJ
  TITLE     EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in
            microtubule stabilization and mechanisms of targeting to 
            microtubules.
  JOURNAL   Mol Biol Cell. 2002 Oct . 13(10):3614-26.
  PUBMED    12388761
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2002 Oct . 13(10):3614-26."
COMMENT     Sequence Label: human EB1 in PET28a for protein expression
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gatatcgaat tcccaagaat tcttaatact cttcttgctc ctcctgtggg cccccttcat
      121 caggtatcac aaagccttca tctgtggcat acagaatgtc tacaatcctc tgcaatacag
      181 ggtcgttttc cccctcgttc tcctggcaaa tcaattcaat gttccgtagc tttccgaagt
      241 agaaatccct ctctttctcc aagtcttcaa cagtaagttt caatacgttg acctgctgca
      301 tcaactcagc tgcctcgtcg tctccgttgc ccacaccagg gttctttcgc accacaccag
      361 ggccagcctt aggagccgca gcggttctct gtgttgagat gggcctctgg ggagctgcac
      421 tgctagaagt gagaggtttc ttcggtttat tcagagctgg agcaacaagg gaaggagcca
      481 ctgcagtttc ttgaccttgt ctagcagcca cagggtcata gtcttttcca tcatagtttg
      541 catcgaaaaa cttcttgaac cactgaacga attcaaaatt gtcctgaaac tttcctttta
      601 ctaatttgtc cacaggaatt attttgtcaa cacccattct cttaaaacct gcttgtagta
      661 ttttgaagtt ctggatgtac tcgtgttcta gcttagcttg gaatttcact ttcttcaagg
      721 caatggagcc agggaacagc atgtccataa actgacaata cgcagcccct gagcacaact
      781 gttcgatctt tgtcagattc aactgcagag actcattgat
//
