LOCUS       sequence-51250-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     Tirnauer lab EB1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: human EB1 in pEGFP N1 to express EB1-GFP (JB131)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgatactct tcttgctcct
       61 cctgtgggcc cccttcatca ggtatcacaa agccttcatc tgtggcatac agaatgtcta
      121 caatcctctg caatacaggg tcgttttccc cctcgttctc ctggcaaatc aattcaatgt
      181 tccgtagctt tccgaagtag aaatccctct ctttctccaa gtcttcaaca gtaagtttca
      241 atacgttgac ctgctgcatc aactcagctg cctcgtcgtc tccgttgccc acaccagggt
      301 tctttcgcac cacaccaggg ccagccttag gagccgcagc ggttctctgt gttgagatgg
      361 gcctctgggg agctgcactg ctagaagtga gaggtttctt cggtttattc agagctggag
      421 caacaaggga aggagccact gcagtttctt gaccttgtct agcagccaca gggtcatagt
      481 cttttccatc atagtttgca tcgaaaaact tcttgaacca ctgaacgaat tcaaaattgt
      541 cctgaaactt tccttttact aatttgtcca caggaattat tttgtcaaca cccattctct
      601 taaaacctgc ttgtagtatt ttgaagttct ggatgtactc gtgttctagc ttagcttgga
      661 atttcacttt cttcaaggca atggagccag ggaacagcat gtccataaac tgacaatacg
      721 cagcccctga gcacaactgt tcgatctttg tcagattcaa ctgcagagac tcattgatcc
      781 aggccagcat gtcatgtcga cttaggttat cactggtcac tgac
//
