LOCUS       sequence-51251-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Yu J, de Belle I, Liang H, Adamson ED
  TITLE     Coactivating factors p300 and CBP are transcriptionally 
            crossregulated by Egr1 in prostate cells, leading to divergent 
            responses.
  JOURNAL   Mol Cell. 2004 Jul 2. 15(1):83-94.
  PUBMED    15225550
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jul 2. 15(1):83-94."
COMMENT     Sequence Label: pcDNA3-Egr1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             150..173
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca
      121 gtgtgctgga attctgcaga tatcttatgg actacaagga cgacgatgac aaagtcaagc
      181 ttgcagcggc caaggccgag atgcaattga tgtctccgct gcagatctct gacccgttcg
      241 gctcctttcc tcactcaccc accatggaca actaccccaa actggaggag atgatgctgc
      301 tgagcaacgg ggctccccag ttcctcggtg ctgccggaac cccagagggc agcggcggta
      361 atagcagcag cagcaccagc agcgggggcg gtggtggggg cggcagcaac agcggcagca
      421 gcgccttcaa tcctcaaggg gagccgagcg aacaacccta tgagcacctg accacagagt
      481 ccttttctga catcgctctg aataatgaga aggcgatggt ggagacgagt tatcccagcc
      541 aaacgactcg gttgcctccc atcacctata ctggccgctt ctccctggag cccgcaccca
      601 acagtggcaa cactttgtgg cctgaacccc ttttcagcct agtcagtggc ctcgtgagca
      661 tgaccaatcc tccgacctct tcatcctcgg cgccttctcc agctgcttca tcgtcttcct
      721 ctgcctccca gagcccgccc ctgagctgtg ccgtgccgtc caacgacagc agtcccatct
      781 actcggctgc gcccaccttt cctactccca acactgacat ttttcctgag ccccaaagcc
      841 aggcctttcc tggctcggca g
//
