LOCUS       sequence-51256-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAGI1
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             429..446
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(499..517)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      513..560
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(684..703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatggaact gattaccgtt
      121 catatcgtga aaggccctat gggatttggc ttcaccatcg ccgattcccc gggtggtggt
      181 ggccagcgtg tgaagcaaat tgtagatagc ccgcgctccc gcggcctgaa agaaggcgac
      241 ctgatcgtag aagttaataa aaagaacgtg caagctctga cccataacca ggtagtagat
      301 atgctggtag aatcacctaa gggctccgaa gtgaccctgc tggttcagcg ccagacccgt
      361 ctgtgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg
      421 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      481 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      541 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      601 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      661 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      721 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      781 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      841 gccctgatag acggtttt
//
