LOCUS       sequence-51279-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Spangle JM, Munger K
  TITLE     The human papillomavirus type 16 E6 oncoprotein activates mTORC1 
            signaling and increases protein synthesis.
  JOURNAL   J Virol. 2010 Sep;84(18):9398-407. Epub 2010 Jul 14.
  PUBMED    20631133
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2010-09"; volume: "84(18)"; pages: "9398-407. Epub 2010 Jul 
            14"
COMMENT     Sequence Label: pNCMV
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..184
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             197..223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(325..344)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    390..445
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(444..463)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
       61 ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct
      121 gtctcatcat tttggcaaag aattcctcga cggatctatg gactacaagg atgacgatga
      181 caagctcgat ggaggatacc catacgatgt tccagattac gctgatatcg gatccgtcga
      241 ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
      301 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
      361 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
      421 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
      481 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
      541 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
      601 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
      661 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
      721 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacgg
      781 atcggccgca
//
