LOCUS       sequence-51289-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Shen X, Park JS, Qiu Y, Sugar J, Yue BY
  TITLE     Effects of Sp1 overexpression on cultured human corneal stromal 
            cells.
  JOURNAL   Genes Cells. 2009 Oct;14(10):1133-9. Epub 2009 Sep 16.
  PUBMED    19758310
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells."; date: "2009-10"; volume: "14(10)"; pages: "1133-9. Epub 
            2009 Sep 16"
COMMENT     Sequence Label: pEGFP-Sp1
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(67..270)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(96..116)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        271..574
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(829..848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtagcgc tagcggatct gacggttcac
       61 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      121 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      181 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      241 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      301 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      361 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      421 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      481 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      541 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      601 taatgacccc gtaattgatt actattaata actaatgcat ggcggtaata cggttatcca
      661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgt
//
