LOCUS       sequence-51293-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1559
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     196..214
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    235..253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      677..763
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(798..815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccaccttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc
       61 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
      121 accttaccag agggcgcccc agctggcaat tccgacgtct aagaaaccat tattatcatg
      181 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagtcccta
      241 tcagtgatag agattgacat ccctatcagt gatagagata ctgtcgacga gtcagacaac
      301 gaagtgaaca tcaggatgat gacacttctg caggacacac caggatggtg tttcagggaa
      361 aggcttctgg atgaagcgaa gaggatgacg caggacgcgt taaaggacac ctccaggatg
      421 gagaatgaga accggtcagg atgattcggt gggtcaggaa ggccagggac acttcaggat
      481 gaagtatcac atcggggtgg tgtgagcagg aagcaatagt tcaggatgaa cgattggccg
      541 caaggccaga ggaaaagttg tcaaggatga gcagggagca acaaaagtag ctggaatgct
      601 gcgaaacgaa ccgggagcgc tgtgaataca gtgctccctt tttttattgg gcccatggta
      661 cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      721 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta
      781 gggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
      841 gcg
//
