LOCUS       sequence-51296-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1561
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    230..248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    255..273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(294..312)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(545..564)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 aagcttcttt caggtacctt agtaactgga ctgctgggat ttttcagcct ggatacgctg
       61 gtagatctct tcacggtgaa cagaaacttc cttcggggca tttacgccaa tacgtacctg
      121 gttgcccttt acccctaaaa ctgtcacggt gacctcatcc ccaatcatga gggtctcacc
      181 aactcgacga gtcagaatca gcattttttt tcctccttac ccgggagtat ctctatcact
      241 gatagggatg tcaatctcta tcactgatag ggactcgagg tgaagacgaa agggcctcgt
      301 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcggaatt
      361 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
      421 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg
      481 atcgtttcgc atgattgaac aaggtggatt gcacgcaggt tctccggccg cttgggtgga
      541 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
      601 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
      661 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
      721 cgcagctgtg ctcgacgttg tcactgaagc gggnaaggga ctggctgcta ttgggcgaag
      781 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaa
//
