LOCUS       sequence-51306-e         449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 449)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BTBD6
FEATURES             Location/Qualifiers
     source          1..449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt gtagatctgg
       61 gtaccgagaa cctgtacttc caatccatgt tcaacaatga gctcatggcc gacgtgcact
      121 tcgtcgtggg gcccccgggg gcgaccagga cggtgcccgc ccacaagtac gtcttggctg
      181 tcggcagctc cgtcttctat gccatgttct acggagacct ggcggaagtc aaatctgaaa
      241 ttcacattcc agacgtggag cccgcagcct ttctgatcct cttaaagtac atgtacagtg
      301 atgagatcga tctggaagcc gacacggtgc tggccactct gtatgctgct aagaagtaca
      361 tcgtcccagc attggcaaaa gcctgtgtca actttctgga gacaagtttg tgacagtaaa
      421 ggtggatacg gatccgaatt cgagctccg
//
