LOCUS       sequence-51331-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression vector with Gateway cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCXLE-gw
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..180
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(221..526)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(323..342)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttgtaatcca gaggttgatt atcatatgac tagtccccga agcttgaatt atcgaaccac
       61 tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      121 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
      181 gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
      241 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
      301 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
      361 tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt gcactggcca
      421 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
      481 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
      541 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
      601 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
      661 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
      721 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
      781 catatcagt
//
