LOCUS       sequence-51348-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Scheffner M, Huibregtse JM, Howley PM
  TITLE     Identification of a human ubiquitin-conjugating enzyme that mediates
            the E6-AP-dependent ubiquitination of p53.
  JOURNAL   Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801.
  PUBMED    8090726
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1994-09-13"; volume: "91(19)"; pages: 
            "8797-801"
COMMENT     Sequence Label: p3993 pET ubcH7
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             490..512
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(543..562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(544..562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(646..665)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgttagcagc cggatcctta gtccacaggt cgcttttccc catatttctt tgtaaactct
       61 tcagcattct tacagaattt tttacggtcc ttagagtatt cttcagctag gtcagcccga
      121 agcgggtgct caggctgggg gtcattcacc agtgctatga gggactggat tacttggtcg
      181 gttttggttg ctggcttcca gttttcggca ctaattactg gcagacagac ctgccccttt
      241 tcgtcgatgt ttgggtgata gatctttgtt ttaaatgtga tcttcggtgg tttgaatggg
      301 tactctgctg gaaagttgat ttcgattctg aaggctccct tatcatatgg agggttgtca
      361 ggaacaataa gcccttgcca agtcaataaa ttagcttcat caacctggat gttacggaag
      421 tttttcatcc cacatttgcg gatttcttca agctccttca tcagcctcct gctggccgcc
      481 atatgtatat ctccttctta aagttaaaca aaattatttc tagagggaaa ccgttgtggt
      541 ctccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac
      601 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
      661 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
      721 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
      781 tccttgcggc ggcggtgctc aacggcctca acctactact ggggctgctt ct
//
