LOCUS       sequence-51357-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ARHGAP15
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(712..732)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(757..774)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             785..807
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    822..846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(846..865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(847..865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtgtgtgctc gagtgcggcc gcaagcttgt cgacggagct cgaattcgga tccgtatcca
       61 cctttactgt cactctgagc cgaagatctt actgtactca ctcagcatga gctcagctat
      121 ctggttctgg tagaccatgt ggatcgccat gtttcctgtt tcattttcag ctcgcagaag
      181 ggtaggtcca aatacaatcc ccaagctttg cgtggacatg aggttcttgg aggctttggc
      241 cactatctta gttagatgtc caaagaggac tttcatggtg tcacgatttg gcggagggag
      301 tttttgtaca agagatttta cagcttcaat tcttgtgttg ttgtcttgct ttttgatcgc
      361 ttccacaaac tgctcaaaga aactgtaagg gaagagcggc tcaggcagct cccggaaaaa
      421 catcttcagt gctccggtga caacgtggat gtcctcccac tggctgtcgt ccaaattcag
      481 cttctcttct tggttgacaa taaatcttaa cttctgtatt gttgccagat tgccactaac
      541 tcgatatatt ccatcaacat ctagacctct tttctcaaca gcttcaatgc attgctttac
      601 aaaccacgga actgtggaat tttcacgttc acacactttg tgcagatgag agccaaaaat
      661 ttgatcttta ataagtcctt tttcttgcag agttttcagg gaaggtctca tggattggaa
      721 gtacaggttc tcggtaccca gatctacacc agaagaatga tgatgatgat ggtgcatatg
      781 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      841 aattccccta tagtgagtcg tattaa
//
