LOCUS       sequence-51366-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTPN4
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..36
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             399..416
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(469..487)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      483..530
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(655..674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtagatctgg gtaccgagaa cctgtacttc caatccatgg ataatcttgt cctaatcaga
       61 atgaaacctg atgaaaatgg gaggtttgga ttcaatgtaa agggaggata tgatcagaag
      121 atgcctgtga ttgtgtctcg agtagcacca ggaacacctg ctgacctctg tgtccctaga
      181 ctgaatgaag gggaccaagt tgtactgatc aatggtcggg acattgcaga acacactcat
      241 gatcaggttg tgctgtttat taaagctagt tgtgagagac attctgggga actcatgctt
      301 ctagttcgac ctaatgctgt agaaagcacc gtttgacagt aaaggtggat acggatccga
      361 attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag
      421 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      481 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      541 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      661 tcgctttctt cccttccttt ctcgccacgt tcgccgggct ttccccgtca agctctaaat
      721 cgggggctcc cttttagggt tccgattt
//
