LOCUS       sequence-51371-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TTK
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..19
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             44..64
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcaccatcat catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca
       61 atccatgtcg gttaaaggaa gaatttattc catattaaag cagataggaa gtggaggttc
      121 aagcaaggta tttcaggtgt taaatgaaaa gaaacagata tatgctataa aatatgtgaa
      181 cttagaagaa gcagataacc aaactcttga tagttaccgg aacgaaatag cttatttgaa
      241 taaactacaa caacacagtg ataagatcat ccgactttat gattatgaaa tcacggacca
      301 gtacatctac atggtaatgg agtgtggaaa tattgatctt aatagttggc ttaaaaagaa
      361 aaaatccatt gatccatggg aacgcaagag ttactggaaa aatatgttag aggcagttca
      421 cacaatccat caacatggca ttgttcacag tgatcttaaa ccagctaact ttctgatagt
      481 tgatggaatg ctaaagctaa ttgattttgg gattgcaaac caaatgcaac cagatacaac
      541 aagtgttgtt aaagattctc aggttggcac agttaattat atgccaccag aagcaatcaa
      601 agatatgtct tcctccagag agaatgggaa atctaagtca aagataagcc ccaaaagtga
      661 tgtttggtcc ttaggatgta ttttgtacta tatgacttac gggaaaacac catttcagca
      721 gataattaat cagatttcta aattacatgc cataattgat cctaatcatg aaattgaatt
      781 tcccgatatt ccagagaaag atcttcaaga tgtgttaaag tgttgtttaa a
//
