LOCUS       sequence-51373-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Cook, Jean
  TITLE     Huwe1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR1A-Huwe1
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(458..557)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(576..599)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(607..634)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(726..812)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gatcttttgc taaatacata taggagattg aggactgcca gcaccacttg catatcagag
       61 gaagccaata aagttgtcaa atgctctatg gaactgtaca gatgccggga aaagctgtac
      121 tcaatgagca aggctgtgaa gttcaacaca gccaagagaa gcattttcag ttgctctctt
      181 tctggcctat cacatacgag catccatgac atattctcca ctgtctgtcc agcatctgcc
      241 agtattccat cgaagcggtc caacaggtcc acccagtgat ataactcgca ctttccaatg
      301 ttccatgttt tgatctgctg cagttccaag agaagttgct catcattaca aactttgagt
      361 ttgtctatta aggctctgca gtctgcagga gcctcagtag gtgtcttctt cagtttagtc
      421 ctgtctactt tcatgtcgac tgaattggtt cctttaaagc ctgctttttt gtacaaagtt
      481 ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      541 aataaaatca ttatttgggg cccgagctta agtaactaac taacaggaag agtttgtaga
      601 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
      661 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
      721 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      781 cagtcttccg actgagcctt tcgttttatt tg
//
