LOCUS       sequence-51378-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Ritchie TK, Grinkova YV, Bayburt TH, Denisov IG, Zolnerciks JK, 
            Atkins WM, Sligar SG
  TITLE     Chapter 11 - Reconstitution of membrane proteins in phospholipid 
            bilayer nanodiscs.
  JOURNAL   Methods Enzymol. 2009;464:211-31. doi: 
            10.1016/S0076-6879(09)64011-8.
  PUBMED    19903557
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2009"; volume: "464:211-31. doi"; pages: " 
            10.1016/S0076-6879(09)64011-8"
COMMENT     Sequence Label: pMSP1E3D1_D73C
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttccctctag aatattttgt ttaactttaa gaaggagata taccatgggt catcatcatc
       61 atcatcacga ttatgatatt cctactactg agaatttgta ttttcagggt tctaccttca
      121 gtaaacttcg cgaacaactg ggccccgtga cgcaggaatt ttggtgcaac ctggaaaaag
      181 aaaccgaggg actgcgtcag gaaatgtcca aagatttaga agaggtgaag gccaaggttc
      241 agccatatct cgatgacttt cagaaaaaat ggcaggaaga gatggaatta tatcgtcaaa
      301 aggtggaacc gctgcgtgcg gaactgcaag agggggcacg ccaaaaactc catgagctcc
      361 aagagaagct cagcccatta ggcgaagaaa tgcgcgatcg cgcccgtgca catgttgatg
      421 cactccggac tcatttggcg ccatatctcg atgactttca gaaaaaatgg caggaagaga
      481 tggaattata tcgtcaaaag gtggaaccgc tgcgtgcgga actgcaagag ggggcacgcc
      541 aaaaactcca tgagctccaa gagaagctca gcccattagg cgaagaaatg cgcgatcgcg
      601 cccgtgcaca tgttgatgca ctccggactc atttggcgcc gtattcggat gaacttcgcc
      661 agcgtttggc cgcacgtctc gaggcgctga aagaaaacgg gggtgcccgc ttggctgagt
      721 accacgcgaa agcgacagaa cacctgagca ccttgagcga aaaagcgaaa ccggcgctgg
//
