LOCUS       sequence-51384-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Tritsch NX, Ding JB, Sabatini BL
  TITLE     Dopaminergic neurons inhibit striatal output through non-canonical 
            release of GABA.
  JOURNAL   Nature. 2012 Oct 11;490(7419):262-6. doi: 10.1038/nature11466. Epub 
            2012 Oct 3.
  PUBMED    23034651
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11466"; journalName: "Nature"; date: "2012-10-11- 
            11"; volume: "490"; issue: "7419"; pages: "262-6"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-VGAT-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..82)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(133..166)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      183..192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat
       61 agcatacatt atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct
      121 gggttaaggc aaataacttc gtataggata ctttatacga agttatgcta gcgctaccgg
      181 tcgccaccat ggccaccctg ctccgcagca agctgaccaa tgtggccacc tccgtgtcca
      241 acaagtccca ggccaaggtg agcggcatgt ttgccaggat ggggtttcag gcggccaccg
      301 atgaggaagc ggtgggcttc gcgcactgcg acgatctcga ctttgagcat cgccagggcc
      361 tgcagatgga catcctgaaa tcggaaggcg agccctgcgg agacgagggc gcagaagctc
      421 ccgtcgaggg agacattcat tatcagcgcg gcggcgctcc tctgccaccc tctggctcca
      481 aggaccaggc cgtgggagct ggtggggagt tcgggggtca cgacaaaccc aagatcacgg
      541 cgtgggaagc gggctggaac gtgacaaatg ccattcaggg catgttcgtg ctgggcctac
      601 cctacgccat cctccacggc ggctacctgg ggttgttcct catcatcttc gccgcagtgg
      661 tgtgctgcta caccggcaag atcctcatcg cgtgcctgta cgaggagaac gaagac
//
