LOCUS       sequence-51386-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Tritsch NX, Ding JB, Sabatini BL
  TITLE     Dopaminergic neurons inhibit striatal output through non-canonical 
            release of GABA.
  JOURNAL   Nature. 2012 Oct 11;490(7419):262-6. doi: 10.1038/nature11466. Epub 
            2012 Oct 3.
  PUBMED    23034651
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11466"; journalName: "Nature"; date: "2012-10-11- 
            11"; volume: "490"; issue: "7419"; pages: "262-6"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-VMAT2-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..82)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(133..166)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      189..198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             489..518
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat
       61 agcatacatt atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct
      121 gggttaaggc aaataacttc gtataggata ctttatacga agttatgcta gcgctaccgg
      181 tcgccaccgc caccatggcc ctgagcgatc tggtgctgct gcgatggctg cgggacagcc
      241 gccactcgcg caagctgatc ctgttcatcg tgttcctcgc gctgctgcta gacaacatgc
      301 tgctcaccgt cgtagttccc atcattccca gctacctgta cagcattaag cacgagaaaa
      361 atactacgga aatccagact gccaggccag cgctcacagc ctccacttcc gaaagcttcc
      421 acagcatctt ctcttactat aacaactcta ctgtgttcac ggggaatgcc acggggggcc
      481 tcccaggaga acaaaaactt atttctgaag aagatctggg ggagtcaccc aaggctacca
      541 ccacacagca cactgtgacc aacacgactg tccctcccga ctgccccagt gaagacaaag
      601 accttctaaa tgagaatgtg caagttgggc tgctgtttgc ctccaaagcc actgtccagc
      661 tcctcaccaa cccattcata ggacttctaa ccaacagaat tggctatcca attcccatgt
      721 ttgctggatt ctgcattatg tttatctcaa cagttatgtt tgccttctcc agcagctat
//
