LOCUS       sequence-51392-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Hagting A, Den Elzen N, Vodermaier HC, Waizenegger IC, Peters JM, 
            Pines J
  TITLE     Human securin proteolysis is controlled by the spindle checkpoint 
            and reveals when the APC/C switches from activation by Cdc20 to 
            Cdh1.
  JOURNAL   J Cell Biol. 2002 Jun 24;157(7):1125-37. Epub 2002 Jun 17.
  PUBMED    12070128
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2002-06-24"; volume: "157(7)"; pages: "1125-37. Epub 
            2002 Jun 17"
COMMENT     Sequence Label: pEYFP-Pds1(434)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(732..831)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(761..781)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgaatatct atgtcacagc
       61 aaacaggtgg caattcaaca tccagggtcg acagaatgct tgaaggagac tgcaacagat
      121 tggattccca tggtggagag ggcatcttca caggtgaagg ggggcccagc tgaaacagct
      181 tttcaagctc tctctcctcg tcaaggatca tgagaggcac tccactcaag gggaggtgcg
      241 caatctggtg ctcttcaggc aggtcaaaac tctcaaagtc tagaggattg aagggaaaga
      301 atttttctat ttctggatag gcatcatctg aggcaggaac agagcttttt gctttaacag
      361 tcttctcagt catctttttg gcagaaaagc ttggctgttt ttgtttgagg ggtcccttgg
      421 tctttacaga cttttctgta gctctgttga cagttcccaa agcctttcta gtagctttag
      481 gtaaggctgg tggggcatcg aacgttttgc caaaacgtgg tgttgaaact tgagatctcc
      541 catctaaggc tttgattgaa ggtccagacc ccagcttcag cccatcctta gcaaccacac
      601 gggtgcctgg ttctccattt tccttatcaa catagatcag agtagccatg gtaccgtcga
      661 ctgcagaatt cgaagcttga gctcgagatc tgagtccggt agcgctagcg gatctgacgg
      721 ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      781 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      841 aaactcccat
//
