LOCUS       sequence-51411-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MYNN
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..34
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             445..462
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(515..533)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      529..576
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(700..719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agatctgggt accgagaacc tgtacttcca atccatgtcg caccactgtg agcacctttt
       61 agagagactg aacaaacagc gggaagcagg ttttctctgt gactgtacca tagtgattgg
      121 ggaattccag tttaaagctc ataggaatgt gctggcctcc tttagtgagt attttggtgc
      181 gatctacaga agcacttctg agaacaatgt ctttcttgat cagagtcagg tgaaggctga
      241 tggatttcag aaactgttgg agtttatata cacaggaact ttaaatcttg acagttggaa
      301 tgttaaagaa attcatcagg ctgctgacta tctcaaagtg gaagaggtgg tcactaaatg
      361 caaaataaag atggaagatt gacagtaaag gtggatacgg atccgaattc gagctccgtc
      421 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      481 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      541 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      601 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      661 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      721 cctttctcgc cacgttcgc
//
