LOCUS       sequence-51420-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ECH1
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(918..938)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(963..980)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct tgtcgacgga
       61 gctcgaattc ggatccgtat ccacctttac tgtcaggttt tcagttcctt gttctcagtc
      121 gtggcctgga ccgacttcac gaggtcttgg gtctgcagca tgctcatgtt ccaggacgcc
      181 acgtagttga ggctctcggc caccgaatgg tcgcgggaat acagcaggtt gaccttggtg
      241 ctctgcaccg ccacggggct cttgctggaa atctcggccg ccagcgctaa ggcagcatcc
      301 agcatgacct ctttgtctgg gaacacccgg ctgaccagcc cactgcccag ggcctcgtca
      361 gccatcatct tgcgggcggt gaaggccagc tcgttgacca ggctctggtt cccgatgacc
      421 ttgggcaggc gctgcagtgt tcctacatcg gcagccaaac ccacgtccac ctccttcacc
      481 tggaagaaag catcctgggc acagtaccgg atgtcacagg cggtgacaag gtccacacct
      541 ccgccaatgc agcccccatg gacggcagca atcacgggct tggggcacct ctcgatgacg
      601 ttgaaggtct cctggtatcg agtgatgatg tcacggaggt accagctgat ccgggccaca
      661 tcatctcctt tgggctgcag gatgtctgaa gccatgtcca tcaggtcaat acctgcagtg
      721 aacatttttc ctgcaccaga gatcaccacc gcccgacagt cagcgtctct cgaaatcttg
      781 ttgaagcact ctaccatctc tctccagaag accttgttca tggcattcct cttgttgggc
      841 cggttgagct ggacatgcag aacatgtttc tgcgcagacg tcacacgaag ggactcatag
      901 ctgtggtctg gggccatgga ttggaagtac aggttctcgg tacccagatc tacaccagaa
      961 gaatgatgat gatgatggtg cat
//
