LOCUS       sequence-51422-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ECH1
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..82
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 agaaggagat atacatatgc accatcatca tcatcattct tctggtgtag atctgggtac
       61 cgagaacctg tacttccaat ccatggcccc agaccacagc tatgagtccc ttcgtgtgac
      121 gtctgcgcag aaacatgttc tgcatgtcca gctcaaccgg cccaacaaga ggaatgccat
      181 gaacaaggtc ttctggagag agatggtaga gtgcttcaac aagatttcga gagacgctga
      241 ctgtcgggcg gtggtgatct ctggtgcagg aaaaatgttc actgcaggta ttgacctgat
      301 ggacatggct tcagacatcc tgcagcccaa aggagatgat gtggcccgga tcagctggta
      361 cctccgtgac atcatcactc gataccagga gaccttcaac gtcatcgaga ggtgccccaa
      421 gcccgtgatt gctgccgtcc atgggggctg cattggcgga ggtgtggacc ttgtcaccgc
      481 ctgtgacatc cggtactgtg cccaggatgc tttcttccag gtgaaggagg tggacgtggg
      541 tttggctgcc gatgtaggaa cactgcagcg cctgcccaag gtcatcggga accagagcct
      601 ggtcaacgag ctggccttca ccgcccgcaa gatgatggct gacgaggccc tgggcagtgg
      661 gctggtcagc cgggtgttcc cagacaaaga ggtcatgctg gatgctgcct tagcgctggc
      721 ggccgagatt tccagcaaga gccccgtggc ggtgcagagc accaaggtca acctgctgta
      781 ttcccgcgac cattcggtgg
//
