LOCUS       sequence-51423-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SNTB2
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(395..415)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(440..457)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             468..490
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    505..529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(529..548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(530..548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(632..651)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttctttggc ttgtagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgga tccgtatcca cctttactgt caaacaacag
      121 tgtttacttc tcggatgaac ttgacctcca gcagcacctc cttgcccgcg cgcttcagcg
      181 cctgcacggc ctggtcgtgg gtggcctggc gcaggtcggt gccgttcacc gacaggatgg
      241 cgtcgcccag ccgcagcgcc cggctctggt cggcagccag cccggggaag atcttggaga
      301 tgaggatcgg catccggttc tcgcggccgc ccttgatgct gatgcccagg ccgcccgcct
      361 cttgcttcac cacccgcacc cggcgcacgg gcatggattg gaagtacagg ttctcggtac
      421 ccagatctac accagaagaa tgatgatgat gatggtgcat atgtatatct ccttcttaaa
      481 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
      541 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
      601 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      661 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      721 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
      781 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
//
