LOCUS       sequence-51429-e         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: KAT2A
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(206..226)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(251..268)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             279..301
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    316..340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(340..359)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(341..359)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(443..462)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agagcttccg ggtcacgtag tagcggcttc gcagccgctc agtcatggtc ttcaggtcaa
       61 tggggaagcg gatgacctcg tagtagtcag gggcctccga cttcttcaca ggctccatga
      121 agggccaggc actggggtga gacttgattt gggccagcag gtttttgagg gttgtgtaga
      181 gctggtcggg gtccttcagc tccatggatt ggaagtacag gttctcggta cccagatcta
      241 caccagaaga atgatgatga tgatggtgca tatgtatatc tccttcttaa agttaaacaa
      301 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      361 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
      421 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
      481 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
      541 gggggactgt tgggcgccat ctccttgcat gcaccattcc
//
