LOCUS       sequence-51432-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GSG2
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gggagaatgt agtcagaagg
      121 gccccgtacc attctcgcac tgcctcccca cggagaaatt gcaacgttgt gagaaaatcg
      181 gtgaaggcgt gtttggtgag gtatttcaga ccattgccga ccatactccg gtcgctatca
      241 agattatcgc gattgaaggc cccgatctgg taaacggtag tcaccaaaag acatttgagg
      301 aaatcttgcc agagattatc atctccaagg aactgtcttt gctgtcaggc gaggtgtgca
      361 atcgcacaga aggtttcatt ggcctgaaca gtgttcattg tgtccagggt tcgtatcctc
      421 cgttgctgtt gaaagcatgg gaccactaca acagcactaa aggctccgcc aatgatcgtc
      481 ccgacttctt caaggatgac caactgttca tcgtgctgga gtttgagttc ggcggcattg
      541 atttggaaca gatgcgcacc aaactctctt cattggctac agcgaagagt atcctgcatc
      601 agttgaccgc cagcctggca gtagctgagg cctccctgcg ttttgaacac cgcgacttgc
      661 attggggtaa cgtgctgttg aagaaaactt cgctgaagaa actgcactat acattgaatg
      721 gcaagtcgtc aaccattcca agttgcggtc tgcaagttag catcattgat tacactttgt
      781 cccgtctgga gcgcgacggc atcgtcgtat tttgcgatgt gtcgatggac gaagatttgt
      841 ttaccggtga cggcgattat cagttcgaca tctaccgcct catgaagaaa gagaacaaca
      901 atcgctgggg agagtatcat ccttactcta
//
