LOCUS       sequence-51436-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ATAD2
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..37)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(490..510)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(535..552)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             563..585
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    600..624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(624..643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(625..643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(727..746)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
       61 cgacggagct cgaattcgga tccgtatcca cctttactgt caacggcttt cctgaatttc
      121 ttcgcacagc tgttcaaaat cttcatccag ttcttcttta ataatcgcat aggcggtatc
      181 gcgcagcgca cacgcacgat gacgaatcag gcgatcgccc ggatcacgat ccggattata
      241 ttccagcgca ttgctgcaga tcagatcaat atcgcgcaga taatctttaa cggtcagata
      301 tttatgcaga tcaattttgc tgataacgct gctcagatcc atcggctgtt taatcacggt
      361 cacataatcc ggcacttcat ccggatcaac cggtttggta aaaacacgaa aacgtttatc
      421 aatcgccaga cgatgggtca cgttgcgcag aaaaatacgc agttcgcgaa aggtatcttc
      481 ttcctgcatg gattggaagt acaggttctc ggtacccaga tctacaccag aagaatgatg
      541 atgatgatgg tgcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg
      601 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
      661 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
      721 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
      781 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
      841 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctacta
//
