LOCUS       sequence-51438-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ATAD2
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             551..568
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(621..639)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      635..682
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(806..825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gcaggaagaa gatacctttc
      121 gcgaactgcg tatttttctg cgcaacgtga cccatcgtct ggcgattgat aaacgttttc
      181 gtgtttttac caaaccggtt gatccggatg aagtgccgga ttatgtgacc gtgattaaac
      241 agccgatgga tctgagcagc gttatcagca aaattgatct gcataaatat ctgaccgtta
      301 aagattatct gcgcgatatt gatctgatct gcagcaatgc gctggaatat aatccggatc
      361 gtgatccggg cgatcgcctg attcgtcatc gtgcgtgtgc gctgcgcgat accgcctatg
      421 cgattattaa agaagaactg gatgaagatt ttgaacagct gtgcgaagaa attcaggaaa
      481 gccgttgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca agcttgcggc
      541 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      601 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      661 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
      721 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      781 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      841 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      901 gctttacggc accctcgacc c
//
