LOCUS       sequence-51440-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX8
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..27)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(594..614)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(639..656)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             667..689
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    704..728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(728..747)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(729..747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(831..850)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt cgacggagct
       61 cgaattcgga tccgtatcca cctttactgt catagatcct ctttcttttt tatgatcact
      121 tgtctaacca gagctgctat gtcaggcctg atgacttcaa tgggctcctc tggcctccag
      181 aacttcacaa cttgaccctc agggttgaca agatacttcc aaaaattcca ccttggttcc
      241 ttctttgaag aatcaacaag aaatctaaat gcaggttctc cttcagatcc tagaatctta
      301 atcttgtgga agatggggaa agttactccg tagttttttc ttgcaaaaga ttctacttcc
      361 ttgcttgggc ggggctccga ttctccaaac tgattgcagg gaaaagccaa cacgctgaag
      421 tgggatggtc caaactcttt gtgcagttcc ttcagcccta agtaatttct gtctgtgagt
      481 tggcagtcac tggccacgtt tacaactagt gaaactttgc ctttatactt ttccagagaa
      541 acagttcttc cttttgcatc cttcacttca aaggcataaa agctgttgat catggattgg
      601 aagtacaggt tctcggtacc cagatctaca ccagaagaat gatgatgatg atggtgcata
      661 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
      721 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc
      781 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
      841 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
      901 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
      961 ac
//
