LOCUS       sequence-51442-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX8
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             653..670
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(723..741)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      737..784
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt gtagatctgg
       61 gtaccgagaa cctgtacttc caatccatga tcaacagctt ttatgccttt gaagtgaagg
      121 atgcaaaagg aagaactgtt tctctggaaa agtataaagg caaagtttca ctagttgtaa
      181 acgtggccag tgactgccaa ctcacagaca gaaattactt agggctgaag gaactgcaca
      241 aagagtttgg accatcccac ttcagcgtgt tggcttttcc ctgcaatcag tttggagaat
      301 cggagccccg cccaagcaag gaagtagaat cttttgcaag aaaaaactac ggagtaactt
      361 tccccatctt ccacaagatt aagattctag gatctgaagg agaacctgca tttagatttc
      421 ttgttgattc ttcaaagaag gaaccaaggt ggaatttttg gaagtatctt gtcaaccctg
      481 agggtcaagt tgtgaagttc tggaggccag aggagcccat tgaagtcatc aggcctgaca
      541 tagcagctct ggttagacaa gtgatcataa aaaagaaaga ggatctatga cagtaaaggt
      601 ggatacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca
      661 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      721 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      781 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      841 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcg
//
