LOCUS       sequence-51446-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DUSP16
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             456..473
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             523..540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(593..611)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      607..654
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(778..797)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaaggagata tactatgatt ggaactcaaa ttgttactga gaggttggtg gctctgctgg
       61 aaagtggaac ggaaaaagtg ctgctaattg atagccggcc atttgtggaa tacaatacat
      121 cccacatttt ggaagccatt aatatcaact gctccaagct tatgaagcga aggttgcaac
      181 aggacaaagt gttaattaca gagctcatcc agcattcagc gaaacataag gttgacattg
      241 attgcagtca gaaggttgta gtttacgatc aaagctccca agatgttgcc tctctctctt
      301 cagactgttt tctcactgta cttctgggta aactggagaa gagcttcaac tctgttcacc
      361 tgcttgcagg tgggtttgct gagttctctc gttgtttccc tggcctctgt gaaggaaaat
      421 ccactctagt ccctacctgc atttctcagc ctgcgcacca tcatcaccac cattgaggat
      481 ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac
      541 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      601 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      661 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      721 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      781 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      841 atcggggggg
//
