LOCUS       sequence-51449-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RHPN2
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(365..385)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(410..427)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             438..460
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    475..499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(499..518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(500..518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(602..621)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcagaaggta acttcgtcca
      121 ggaggctcac gactttcatc tcgatctcgt cctcgccaaa gctcttcagc agcttcataa
      181 cctcactcag cgtcagccac ttacaatcca caagctgaat ggagacaata taatctcctt
      241 cccgggctcc tgccaccgag gcagagcagt aaggatccag gaagtgaacc tgaacggggg
      301 cgttccctct caaggtgaac cccaagtccc cttcttctgc agtgaagcgg atgcttcgag
      361 gcatggattg gaagtacagg ttctcggtac ccagatctac accagaagaa tgatgatgat
      421 gatggtgcat atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt
      481 gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg
      541 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      601 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      661 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      721 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      781 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg
      841 gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtgg
//
