LOCUS       sequence-51452-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RHPN2
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..46
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             373..390
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(443..461)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      457..504
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(628..647)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgc ctcgaagcat
       61 ccgcttcact gcagaagaag gggacttggg gttcaccttg agagggaacg cccccgttca
      121 ggttcacttc ctggatcctt actgctctgc ctcggtggca ggagcccggg aaggagatta
      181 tattgtctcc attcagcttg tggattgtaa gtggctgacg ctgagtgagg ttatgaagct
      241 gctgaagagc tttggcgagg acgagatcga gatgaaagtc gtgagcctcc tggacgaagt
      301 taccttctga cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg
      361 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      421 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      481 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      541 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      601 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      661 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      721 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      781 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaa
//
