LOCUS       sequence-51456-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LNX2
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(317..337)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(362..379)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             390..412
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    427..451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(451..470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(452..470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(554..573)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        779..856
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ctcgaattcg gatccgtatc cacctttact gtcacagttc gatttcgggt ttccctggtc
       61 tagcaattgt taaattcact ctctctccac tggcctgaat aatctgggca gcaagctccg
      121 gagttccata cttcaggtcg tgcccattga tggccagcac tcggtcattg ctgcttagcc
      181 tgccgtcctg ggcagccaac cccccttcca acaggtcaag aataaaaacc cctggctcat
      241 ctgtccttcg caccaattta atgccaagct gttcaccaga gtcccgttta tgaagagcca
      301 cttggagaat ctccatggat tggaagtaca ggttctcggt acccagatct acaccagaag
      361 aatgatgatg atgatggtgc atatgtatat ctccttctta aagttaaaca aaattatttc
      421 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg
      481 atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      541 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      601 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      661 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      721 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga
      781 caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
      841 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgca
//
