LOCUS       sequence-51457-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LNX2
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             446..463
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(516..534)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      530..577
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(701..720)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat ggagattctc caagtggctc
      121 ttcataaacg ggactctggt gaacagcttg gcattaaatt ggtgcgaagg acagatgagc
      181 caggggtttt tattcttgac ctgttggaag gggggttggc tgcccaggac ggcaggctaa
      241 gcagcaatga ccgagtgctg gccatcaatg ggcacgacct gaagtatgga actccggagc
      301 ttgctgccca gattattcag gccagtggag agagagtgaa tttaacaatt gctagaccag
      361 ggaaacccga aatcgaactg tgacagtaaa ggtggatacg gatccgaatt cgagctccgt
      421 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      481 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      541 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      601 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      661 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      721 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      781 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta ggg
//
