LOCUS       sequence-51469-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Nilsson J, Yekezare M, Minshull J, Pines J
  TITLE     The APC/C maintains the spindle assembly checkpoint by targeting 
            Cdc20 for destruction.
  JOURNAL   Nat Cell Biol. 2008 Dec . 10(12):1411-20.
  PUBMED    18997788
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Dec . 10(12):1411-20."
COMMENT     Sequence Label: pFAST-BAC-Cdc20wt (2193)
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             96..113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             135..155
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtacgttttg tataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg
       61 ggcgcggatc tcggtccgaa accatgtcgt actaccatca ccatcaccat cacgattacg
      121 atatcccaac gaccgaaaac ctgtattttc agggcgccat gggatctcga gctcaagctt
      181 cgaattctat ggcccaattt gcctttgaat ccgatcttca tagcctcttg caactcgacg
      241 cccctattcc taacgcccct cccgccaggt ggcaaaggaa ggctaaagag gctgccgggc
      301 ctgctcctag ccctatgaga gctgctaata ggagtcattc tgctgggcgc acccccggga
      361 ggacccccgg gaagagttcc agtaaagtgc aaacaacccc ctctaagccc ggtggcgata
      421 ggtacattcc tcacaggtcc gccgctcaaa tggaagtcgc ttcttttctg ctctctaaag
      481 aaaatcaaag cgagaatagc caaaccccta caaaaaaaga gcaccaaaag gcttgggccc
      541 tcaatctcaa tggcttcgac gtggaagagg ctaagatttt gagattgtcc ggcaagcccc
      601 agaacgcccc cgaaggctac caaaatcggc tcaaggtgtt gtattctcag aaagctaccc
      661 ccgggagttc tagaaaaaca tgtaggtata tccccagtct ccccgatagg attctcgacg
      721 cccccgagat taggaacgat tactatctca atttggtcga ctggtccagc ggcaacgtgc
      781 tcgctgtcgc cctcgataat tccgtctatc tctggtccgc ctctagcggc gatattctcc
      841 aattgctcca gatggaacaa cccggcgagt acatcagtag cgtcgcttgg
//
