LOCUS       sequence-51493-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Martin CD, Rojas G, Mitchell JN, Vincent KJ, Wu J, McCafferty J, 
            Schofield DJ
  TITLE     A simple vector system to improve performance and utilisation of 
            recombinant antibodies.
  JOURNAL   BMC Biotechnol. 2006 Dec 7;6:46.
  PUBMED    17156422
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-12-7"; volume: "6"; pages: "46"
COMMENT     Sequence Label: pSANG14-3F
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(71..136)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(143..160)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg acttattaaa
       61 gcttttatta cttgtcatcg tcatccttgt aatcgatgtc atgatcttta taatcaccgt
      121 catggtcttt gtagtccagc ttgtgatggt gatgatgatg tgcagacggt actttcagcc
      181 ccagagcggc tttcatggtg tagaagagat cggtctggtc ggtcagtcca acaacattgg
      241 cggcatgcgg gccatacgcc gcaatacgca actgactgcc ggtatgttct tgtgaatcct
      301 cttcggagtt cccgtaactc atcaccatca ctgcgccatc tttggtattt agcgcctggg
      361 tgaggcccgg agctttggta tccggcgcaa caatctggct ggcgtgggcg tgatcagcgg
      421 tgactatgac cagcgtgtta ccctcctttt tagcgaattc cagcgcccgt tgtacggctt
      481 catcgagatc gaccgtctcg ccaatttgcc cacaaggatt cgcagcatga ttctgtttat
      541 cgattgacgc accttcaact tgcaggaaaa agcctttctc atttttactc aacaattcaa
      601 tggctttgtc ggtcatctgc gccagggttg gtacactgtc attacgttgc ggatttggcg
      661 tacaggtgac tgcgggctta tcgatattgc cgtggtacgt tgctttcggt cctagccagc
      721 gcactggcat attgccgtca gcaaacagac caagcagggg tttttgctga ttcgcttccg
      781 tcaccgagtt cagtgaggca gcatcgctca cca
//
