LOCUS       sequence-51505-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Itakura E, Mizushima N
  TITLE     Characterization of autophagosome formation site by a hierarchical 
            analysis of mammalian Atg proteins.
  JOURNAL   Autophagy. 2010 Aug;6(6):764-76.
  PUBMED    20639694
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2010-08"; volume: "6(6)"; pages: "764-76"
COMMENT     Sequence Label: pMXs-puro GFP-b5 ER
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(187..207)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        192..521
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      372..507
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(531..550)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tggacgagct gtacaagatc gatgaattga attccccttc ggaaaccctt atcactactg
       61 tcgagtctaa ttccagttgg tggaccaact gggtgatccc agccatctca gccctggtgg
      121 tagctctgat gtatcgcctc tacatggcag aagattaagc ggccgccagc acagtggtcg
      181 acctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
      241 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
      301 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
      361 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      421 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
      481 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttacc atgaccgagt
      541 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
      601 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
      661 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
      721 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
      781 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
      841 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cc
//
