LOCUS       sequence-51514-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Tirnauer JS, Grego S, Salmon ED, Mitchison TJ
  TITLE     EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in
            microtubule stabilization and mechanisms of targeting to 
            microtubules.
  JOURNAL   Mol Biol Cell. 2002 Oct . 13(10):3614-26.
  PUBMED    12388761
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2002 Oct . 13(10):3614-26."
COMMENT     Sequence Label: human EB1 in PET28a for protein expression
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             96..128
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcatgact ggtggacagc
      121 aaatgggtcg cggatccatg gcagtgaacg tatactcaac gtcagtgacc agtgataacc
      181 taagtcgaca tgacatgctg gcctggatca atgagtctct gcagttgaat ctgacaaaga
      241 tcgaacagtt gtgctcaggg gctgcgtatt gtcagtttat ggacatgctg ttccctggct
      301 ccattgcctt gaagaaagtg aaattccaag ctaagctaga acacgagtac atccagaact
      361 tcaaaatact acaagcaggt tttaagagaa tgggtgttga caaaataatt cctgtggaca
      421 aattagtaaa aggaaagttt caggacaatt ttgaattcgt tcagtggttc aagaagtttt
      481 tcgatgcaaa ctatgatgga aaagactatg accctgtggc tgctagacaa ggtcaagaaa
      541 ctgcagtggc tccttccctt gttgctccag ctctgaataa accgaagaaa cctctcactt
      601 ctagcagtgc agctccccag aggcccatct caacacagag aaccgctgcg gctcctaagg
      661 ctggccctgg tgtggtgcga aagaaccctg gtgtgggcaa cggagacgac gaggcagctg
      721 agttgatgca gcaggtcaac gtattgaaac ttactgttga agacttggag aaagagaggg
      781 atttctactt cggaaagcta cggaacattg aattgatttg ccaggagaac gagggggaaa
      841 acgaccctgt attgcagagg attgta
//
