LOCUS       sequence-51518-e         490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pMADM-alpha
FEATURES             Location/Qualifiers
     source          1..490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    35..68
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(123..142)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(204..226)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ttgctcacca tggtggcgga tatcggatcc cggggaagtt cctattctct ataaagtata
       61 ggaacttcaa attcgatggg cccgcggtat caaatcaatt ctttgccaaa atgatgagac
      121 agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg
      181 ttagcagagg ctctagagcc gccggtcaca cgccagaagc cgaaccccgc cctgccccgt
      241 cccccccgaa ggcagccgtc cccccgcgga cagccccgag gctggagagg gagaagggga
      301 cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac
      361 cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc tcccagattt cggctccgca
      421 cagatttggg acaaaggaag tccctgcgcc ctctcgcacg attaccataa aaggcaatgg
      481 ctgcggctcg
//
