LOCUS       sequence-51520-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   MacBeath G, Kast P
  TITLE     UGA read-through artifacts--when popular gene expression systems 
            need a pATCH.
  JOURNAL   Biotechniques. 1998 May;24(5):789-94.
  PUBMED    9591128
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "1998-05"; volume: "24(5)"; pages: "789-94"
COMMENT     Sequence Label: pATCH1
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(97..144)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     140..158
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(227..244)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(277..295)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(316..332)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(316..332)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(329..351)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    340..356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(364..370,371..388,389..394))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    409..430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(547..564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
       61 cccattcgcc aatccggata tagttcctcc tttcagcaaa aaacccctca agacccgttt
      121 agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcca actcagcttc
      181 ctttcgggct ttgttagcag ccggatcagc tgactagtca ttattagtgg tggtggtggt
      241 ggtgctcgag ggggggcccg gtaccagctt ttgttccctt tagtgagggt taatttcgag
      301 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      361 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      421 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      481 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      541 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      781 aaacccgaca
//
