LOCUS       sequence-51522-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ECHDC3
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gggggcgggg cggcgggagt
      121 cggagccgcg gcccaccagc gcgcggcagc tggacggcat aaggaacatc gtcttgagca
      181 atcccaagaa gaggaacacg ttgtcacttg caatgctgaa atctctccaa agtgacattc
      241 ttcatgacgc tgacagcaac gatctgaaag tcattatcat ctcggctgag gggcctgtgt
      301 tttcttctgg gcatgactta aaggagctga cagaggagca aggccgtgat taccatgccg
      361 aagtatttca gacctgttcc aaggtcatga tgcacatccg gaaccacccc gtccccgtca
      421 ttgccatggt caatggcctg gccacggctg ccggctgtca actggttgcc agctgcgaca
      481 ttgccgtggc gagcgacaag tcctcttttg ccactcctgg ggtgaacgtc gggctcttct
      541 gttctacccc tggggttgcc ttggcaagag cagtgcctag aaaggtggcc ttggagatgc
      601 tctttactgg tgagcccatt tctgcccagg aggccctgct ccacgggctg cttagcaagg
      661 tggtgccaga ggcggagctg caggaggaga ccatgcggat tgctaggaag atcgcgtcac
      721 tgagccgtcc ggtggtgtcc ctgggcaaag ccaccttcta caagcagctg ccccaggacc
      781 tggggacggc ttactacctc acctcccagg ccatggtgga caacctggcc ctgcgggacg
      841 ggcaggaggg tatcacggcc ttcctccaga agagaaaacc tgtctggtca cactgacagt
      901 aaaggtgg
//
